A total of 2109 samples have been tested to ensure that the breeding bulls to be used for production of Frozen Semen are devoid of inherited disorders viz., Bovine Leukocyte Adhesion Deficiency (BLAD), Deficiency of Uridine Monophosphate Synthase (DUMPS), Bovine Citrulinemia (BC) and Factor XI deficiency syndrome.
422 bull calves (trios) were verified for parentage to include them as high genetic merit bulls in the semen production programme in the state of Tamil Nadu.
A novel genotyping protocol of High-Resolution Melt (HRM) analysis utilizing RT-PCR was standardized to screen the samples for inherited disorders viz. BLAD and BC.
A total of 200 samples of breeding bulls are screened every year for the presence of any karyological abnormalities before they are inducted for semen production.
Two encapsulated probiotic consortia were developed based on in vitro chicken cytokine profiling studies.
NABL accreditation, in accordance with the ISO/IEC 17025:2017, for the Rabies Diagnostic Laboratory in the Department of Animal Biotechnology, MVC, is further renewed for a period of two years, from 8th December 2021 to 7th December 2023. The scope of accreditation includes rabies antigen detection by Direct Fluorescent Antibody Technique on brain / corneal impression smears from animals and anti-rabies antibody quantification by Fluorescent Antibody Virus Neutralization assay in serum samples from animals.
An indigenous ABT Mini Encapsulator V500 was designed and fabricated to encapsulate large volume of probiotic organisms in the laboratory condition.
Duck Plague Virus glycoprotein genes viz. gC, gD, gE, gG, UL42 and UL55 were cloned and expressed in the prokaryotic system. A field based diagnostic assay viz. latex agglutination test for duck plague virus antigen as well as antibody detection was evolved.
Prevalence of duck plague infection was assessed in Tamil Nadu through molecular epidemiological study.
Bovine digesta from slaughter house waste can be used as an organic fertilizer and a novel method of bioremediation using waste has been achieved.
Complete genome sequence of canine parvovirus 2a new variant from dogs has been carried out.
Molecular characterization of canine adenovirus type 2 from dogs was done.
Based on the selection criteria, the Cubic model, Ali and Schaeffer model and Mitscherlich X Exponential model were identified as the best models for fitting the lactation curves for the Indigenous, Jersey crossbred and Holstein-Friesian crossbred cows, respectively.
Measurement of methane gas production from the cattle was standardized using SF6 technique by gas chromatography. The feeding of balanced ration significantly reduced the methane emission in g / cow / day and g / kg DDMI / day by 24.45 and 24.66 per cent, respectively than unbalanced ration fed to the indigenous dairy cattle under field conditions.
Herbal mixture containing equal proportions of turmeric rhizomes, shikakai pods, ginger rhizomes, betel leaves and guava leaves, feed additive mixture added at 1.50 per cent level of total mixed ration reduced the methane emission by 2.31 per cent on the basis of per kg metabolic body weight and 8.98 per cent on the basis of per kg digestible dry matter intake in indigenous dairy cattle.
Plasmid based reverse genetics system for rescue of Newcastle disease virus (NDV-D58) has been developed. Differentiation of infected and vaccinated birds using a peptide-based screening system has been established.
The combined inactivated vaccine developed using indigenous isolates of Newcastle disease virus (NDV–D58) and Avian Infectious bronchitis virus (IBV-B17) has been adjuvanted with an identified adjuvant from Seppic, France. This vaccine has been subjected to a large-scale controlled field trial in comparison with the regularly used combined inactivated commercial vaccine used in the poultry farms. A close similarity of the indigenous vaccine and the regular commercial vaccine has been demonstrated in terms of seroconversion and transfer of antigen-specific antibodies to yolk.
A total of 120 goat and 103 sheep serum samples were tested by peptide ELISA for PPRV antibodies. Results indicated 95 per cent seropositivity in goats and 93 per cent in sheep samples with correlation values for N (0.787), H (0.858) and cocktail (0.851) peptides in goat and N (0.809), H (0.770) and cocktail (0.772) peptides in sheep. The accuracy, sensitivity and specificity of the standardised N peptide ELISA were found to be 93.2, 93.8 and 85.7 per cent, respectively when compared to the commercial cELISA.
A total of 191 chicken caecal mucosal scraping were collected and screened for the presence of Campylobacter spp. C. coli and C. jejuni which were found to be 28 (54/191), 7 (14/191) 0.5 (1/191) per cent respectively. Two novel sequence types (ST-10872 and ST-11031) of Campylobacter coli isolates were identified.
Five isolates of BTV were isolated from 93 cattle and buffalo blood samples and characterized. Four isolates were identified as serotype 16 and other as serotype 2 based on VP2 gene-based RT-PCR.
Immunogenicity study of inactivated PCV2b and PCV2d vaccines were evaluated by PCV2 serum antibody to capsid protein by ELISA and neutralizing antibody response by SNT. Both the vaccinated groups showed significantly high serum antibody and neutralizing antibody (NA) response for up to 120 dpv in both boosted and non-boosted vaccinated groups, but the levels of serum antibodies started reducing in non-booster groups compared to booster group by 120 dpv in both the vaccine groups.
A total of 73 APEC isolates and 15 avian fecal E. coli (AFEC) isolates were isolated and confirmed by biochemical tests. All the isolates were further confirmed by PCR targeting the Adk gene. Antimicrobial susceptibility profiling indicated that all the APEC and AFEC isolates showed complete resistance to enrofloxacin and colistin sulphate.
Avian Orthoavulavirus1 isolated from both Newcastle disease affected and apparently healthy unvaccinated chicken were subjected to genotypic characterization. These field isolates of Avian Orthoavulavirus1 were subjected to MGB Taqman Real Time PCR assay for pathotyping. The assay identified 16 isolates as virulent. The isolates D217, D230, D223, D209 and D222 were grouped as genotype XIII.2.2 based on phylogenetic trees constructed using the full-length F gene sequences of all representative AOAV-1 genotypes and genotype XIII.
Supply of Newcastle disease virus D58 oral pellet vaccine.
Avian Infectious Bronchitis seed virus (IB 17), recombinant IBV (rIBV), recombinant NDV (rNDV) and Newcastle disease D58 seed virus are maintained and propagated in SPF eggs at different passage levels.
Maintenance of BSR/T7 cell line and B95a cell line for cell banking.
Extraction of outermost vesicle from E.coli isolates for the development of vaccine.
ELISA and Flow through technique using excretory-secretory antigen was standardized and evaluated for the detection of visceral schistosomiasis caused by Schistosomaspindale in cattle.
Detection of Wolbachia in Haemaphysalisbispinosa ticks was reported for the first time in India.
Detection of Babesiaovis and Anaplasmaovis in Rhipicephalushaemaphysaloides tick by molecular methods was reported for the first time in India.
A tick trap employing the push-pull strategy was designed to lure and kill Rhipicephalusmicroplus tick larvae using vapour patches impregnated with pheromones as lures and neem as pushing agent. In trials, 3 per cent of tick larvae were trapped and killed.
Molecular epidemiology of tick borne haemoparasitic diseases in dogs in Chennai.
Assessment of maternal derived antibody and humoral immune response in pups against canine distemper vaccines.
A rapid and sensitive DNA based LAMP assay was standardised for the detection of B. gibsoni in dogs and sensitivity of LAMP was detected up to 10-5 dilution of DNA.
Standardization of assay techniques of select antibiotics in tissue and body fluids using sensitive HPLC.
Development of in-vivo models for disease condition such as anti-diabetic; anti-inflammatory; anti-arthritic; gastric carcinoma; colon cancer, PCOD, etc.
Continuous monitoring of anti-microbial resistance using bacteriological and molecular techniques.
Computation of dosage regimen of antibiotics in chicken by pharmacokinetic studies.
During 2020-22, 24 research projects with a total financial outlay of Rs. 1658.43 lakhs were completed successfully.
At present, 39 externally-funded research projects for the total outlay of Rs. 3283.92 lakhs are functioning in various departments of this institution. A total of 546 research articles have been published by the staff and research scholars of this college which include 160 articles in international journals and 386 in national journals.
The faculty and students presented 183 papers and 53 posters in 134 different seminar and conferences during this period.
Around 83 manuals, 21 books and 78 chapters in books of national and international level were published.
A total of 138 popular articles and three pamphlets were published by the staff for the benefit of farming community.
