CENTRE FOR ANIMAL HEALTH STUDIES

PHARMACOVIGILANCE LABORATORY FOR ANIMAL FEED AND FOOD SAFETY (PLAFFS)

VISION

To prepare and meet out WTO Challenges pertaining to xenobiotic residues (Veterinary drugs, Pesticides and Mycotoxins)
To establish poison control information service

OBJECTIVES

Short-Term Objectives

To have a constant pharmacovigilance on Veterinary drugs (Antibiotics, growth promoters, anthelmenitics) - Drugs which have been banned for use in food animals and Drugs (Antibiotics/Antimicrobials) with MRL

To analyze mycotoxins, pesticides, insecticides, herbicides, minerals, heavy metals in dry and wet samples.

To generate a database on the spectrum of veterinary drugs in feed and feedstuff in Tamil Nadu.

Long-Term Objectives

Traceability /sources of Point contamination
Postmarket surveillance of approved veterinary drugs in livestock products

Quality Policy

Pharmacovigilance Laboratory for Animal Feed and Food Safety (PLAFFS) functioning under the Directorate, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS) is to offer the facility of testing and reporting the levels of Mycotoxins, Drug residues, Pesticides from various feeds, feed stuff and livestock farmers to prepare their products as per International Standards. The service of this laboratory is open to continuous improvement based on feedbacks and findings and through the application of the Quality Policy and Quality objectives.

Salient Findings

T2 toxicosis in ostriches - 2000
Organophosphorous toxicity – 2000
Nitrate/Nitrite toxicosis in equines-2001
Zinc phosphide toxicity in monkeys-2005
Hydrocyanic acid toxicity in cattle-2009
Nitrate/Nitrite toxicity (Weeds) in ponies and cattle-2009
Organocarbamate – Carbofuran toxicity in elephants-2009
Tetradeconic acid toxicity in heifers-2010
Dimethyl pentane in Tilopia fish - 2017
Malathion and Carbofuran toxicity in Broiler Feed - 2017
Glyphosate (Herbicide) toxicity in chicken - 2018
Acetamprid (Pesticide) toxicity in Fish - 2018
Ricinoleic acid and Zinc Phosphide toxicity in cattle feed and feed ingredients - 2018
Glyphosate (Herbicides) toxicity in pelican birds - 2023
Thiram/Thiuram disulfide (Fungicide) in maize - 2023
Metalochlor and Pretachlor (Weedicides) in feed ingredients like maize and soya - 2024

Test Conducted

Analysis of animal feed and food for the presence of the following is being carried out:

Mycotoxins – Aflatoxin, Ochratoxin, Citrinin, Penicillic acid, T2 Toxin, Zearalenone
Pesticides – Organochlorine, Organophosphorous Carbamate & Pyrethroids
Veterinary drugs - Tetracycline, Chloramphenicol, Nitrofurans and Enroflaxacin
Adulterants – Urea
Pharmacotoxicological diagnosis in disease outbreak (Nitrate/Nitrite, Zinc Phosphide, Urea, Hydrocyanic Acid, Gossypol, Ricinoleic acid)

Facilities Available/ Services Offered

Name of the Parameter	Detection method	Charges/Test(Rs.)
Name of the Parameter	Detection method	Students Charges / Test (Rs.)	Tamil Nadu Charges / Test (Rs.)	Outside state Charges / Test (Rs.)
PESTICIDES Organochlorine Organophosphates Organocarbamates Pyrethroids	Gas Chromatography (GC) (Per compound)	800	1200	1300
	Gas Chromatography Mass Spectrometry (GCMS) (Per compound)	750	1200	1300
	Thin Layer Chromatography (TLC)	300	400	500
VETERINARY DRUGS* Chlortetracycline Ciprofloxacin Enrofloxacin Salinomycin Tiamulin Tylosin Chloramphenicol Furazolidone and metabolites	Thin Layer Chromatography (TLC)	300	400	500
	High Performance Liquid Chromatography (HPLC) (Per compound)	800	1200	1300
Aflatoxins (B1, B2, G1, G2, M1 and M2)	Thin Layer Chromatography (TLC)	250	300	350
Aflatoxins (B1, B2, G1, G2, M1 and M2)	High Performance Thin Layer Chromatography (HPTLC) (Per compound)	800	1200	1300
Multimycotoxins (Aflatoxins, Citrinin, Ochratoxin A, T2 Toxin, Zearalenone)	Thin Layer Chromatography (TLC)	400	750	1000
	High Performance Thin Layer Chromatography (HPTLC) (Per compound)	800	1200	1300
Nitrate/Nitrite	Qualitative & Quantitative	300	600	1000
Urea	Qualitative & Quantitative	200	250	300
Zinc Phosphide	Qualitative	300	600	1000
Ricinoleic acid	Gas Chromatography Mass Spectrometry (GCMS)	800	1200	1300
Hydrocyanic acid (HCN)	Qualitative	200	250	300
Gossypol	Qualitative	300	600	1000
Water analysis	Quantitative	300	600	1000
MINERALS (Calcium, Magnesium, Potassium, Sodium, Manganese, Zinc, Selenium, Phosphorus, Sulfur)	Inductively Coupled Plasma Mass Spectrometry (ICPMS)	800	1800	2000
HEAVY METALS (Lead, Mercury, Cadmium, Arsenic, Chromium, Cobalt, Copper, Iron, Nickel, Boron, Aluminium, Silver, Bismuth, Barium, Lithium, Vanadium, Zirconium, Tin, Molybdenum, Antimony, Silicon)	Inductively Coupled Plasma Mass Spectrometry (ICPMS)	1000	2000	2200

*Screening of new/additional drug residue will be analysed based on the standards available

Quantity of Sample required for Analysis

Sample	Quantity/ Numbers
Compounded feed / feed ingredients	250-500grams
Bio-specimens	Minimum 50grams in Saturated Saline
Milk	100ml
Meat	200-250grams
Egg	6-10 Numbers
Fodder	500grams

FACULTY

Dr. A. Sundaresan, Professor and Head
Dr. D. Raghu, Assistant Professor

Professor and Head,
Pharmacovigilance Laboratory for Animal Feed and Food Safety,
Centre for Animal Health Studies, TANUVAS,
Madhavaram Milk Colony, Chennai – 600051.
Phone: +91-44-2555 0111
E-mail: plaffs@tanuvas.org.in