CENTRE FOR ANIMAL HEALTH STUDIES

PHARMACOVIGILANCE LABORATORY FOR ANIMAL FEED AND FOOD SAFETY (PLAFFS)

VISION

To prepare and meet out WTO Challenges pertaining to xenobiotic residues (Veterinary drugs, Pesticides and Mycotoxins)
To establish poison control information service
To obtain NABL accreditation 17025

OBJECTIVES

Short-Term Objectives

To have a constant pharmacovigilance on Veterinary drugs (Antibiotics, growth promoters, anthelmenitics) - Drugs which have been banned for use in food animals and Drugs (Antibiotics/Antimicrobials) with MRL

Drugs which have been banned for use in food animals
Drugs (Antibiotics/Antimicrobials) with MRL

To generate a database on the spectrum of veterinary drugs in feed and feedstuff in Tamil Nadu.

Long-Term Objectives

Traceability /sources of Point contamination
Postmarket surveillance of approved veterinary drugs in livestock products

Quality Policy

Pharmacovigilance Laboratory for Animal Feed and Food Safety (PLAFFS) functioning under the Directorate, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS) is to offer the facility of testing and reporting the levels of Mycotoxins, Drug residues, Pesticides from various feeds, feed stuff and livestock farmers to prepare their products as per International Standards. The service of this laboratory is open to continuous improvement based on feedbacks and findings and through the application of the Quality Policy and Quality objectives.

Salient Findings

T2 toxicosis in ostriches - 2000
Organophosphorous toxicity – 2001
Nitrate/Nitrite toxicosis in equines-2001
Urea toxicity in heifers-2002
Nitrate/Nitrite toxicosis in cattle-2004
Zinc phosphide toxicity in monkeys-2005
Hydrocyanic acid toxicity in cattle-2005
Nitrate/Nitrite toxicity (Weeds) in ponies and cattle-2008
Hydrocyanic acid toxicity (Tapioca) in cattle-2009
Organocarbamate – Carbofuran toxicity in elephants-2009
Tetradeconic acid toxicity in heifers-2010

Test Conducted

Analysis of animal feed and food for the presence of the following is being carried out:

Mycotoxins – Aflatoxin, Ochratoxin, Citrinin, Penicillic acid, T2 Toxin, Zearalenone
Pesticides – Organochlorine, Organophosphorous & Pyrethroids - Veterinary drugs - Banned Veterinary drugs – Chloramphenicol, Nitrofurans, Sulphanamides & Enroflaxacin
Drugs with permissible level – Tetracycline, Macrolide & Betalactam
Adulterants – Melamine/Urea
Pharmacotoxicological diagnosis in disease outbreak (Nitrate/Nitrite, Zinc Phosphide, Urea toxicity, Hydrocyanic Acid toxicity)

Facilities Available/ Services Offered

Name of the Parameter	Detection method	Charges (Rs.)/ Test
Name of the Parameter	Detection method	For Students	For Samples from Tamil Nadu	For Samples from Outside Tamil Nadu
Pesticides: Organocarbamates Organochlorine Organophosphates, Pyrethroids	Gas Chromatography (GC) per Compound	800	1200	1300
	Gas Chromatography Mass Spectrometer (GCMS) per Compound	750	1200	1300
	Thin Layer Chromatography (TLC)	300	400	500
Veterinary Drugs: Chloramphenicol , Furazolidone and metabolites	Liquid Chromatography Mass Spectrometer Mass Spectrometer (LCMS/MS) per Compound	1000	1500	2000
Chlortetracycline Ciprofloxacin Enrofloxacin Salinomycin, Tiamulin, Tylosin	Thin Layer Chromatography (TLC)	300	400	500
	High Performance Liquid Chromatography (HPLC) per Compound	800	1200	1300
	Liquid Chromatography Mass Spectrometer Mass Spectrometer (LCMS/MS) per Compound	1000	1500	2000
Aflatoxins (B1, B2, G1, G2, M1 and M2)	Thin Layer Chromatography (TLC)	250	300	350
Multi mycotoxins	Thin Layer Chromatography (TLC)	400	750	1000
	High Performance Thin Layer Chromatography (HPTLC) per Compound	800	1200	1300
		800	1200	1300
Nitrate/ Nitrite		300	600	1000
Urea	Thin Layer Chromatography (TLC)	200	250	300
FTIR	Fourier transform infrared spectroscopy per compound	150	200	300
ICPMS	Inductively coupled plasma mass spectrometry per compound	1000	1500	2000
LCMS - Time of Flight	Liquid chromatography mass spectrometer per compound	1000	1500	2000

Quantity of Sample required for Analysis

Sample	Quantity/ Numbers
Compounded feed / feed ingredients	250-500grams
Bio-specimens	Minimum 50grams in Saturated Saline
Milk	100ml
Meat	200-250grams
Egg	6-10 Numbers
Fodder	500grams

FACULTY

Dr. Ghadevaru Sarathchandra, Professor and Head
Dr. D. Raghu, Assistant Professor

Professor and Head,
Pharmacovigilance Laboratory for Animal Feed and Food Safety,
Centre for Animal Health Studies, TANUVAS,
Madhavaram Milk Colony, Chennai – 600051.
Phone: +91-44-2555 0111
E-mail: plaffs@tanuvas.org.in