FACULTY OF BASIC SCIENCES

RESEARCH ACHIEVEMENTS

Diagnostic kits developed

Peste des petits ruminants virus (PPRV) antigen detection kit based on flow-through technology using monospecific sera against recombinant nucleoprotein
PPRV antibody detection kit based on lateral-flow technology using recombinant nucleoprotein
CDV-Check-Canine distemper virus (CDV) antibody detection kit based on indirect ELISA using recombinant truncated cross reactive PPRV nucleoprotein
Leptospira antibody detection kits based on flow-through and latex agglutination assays using recombinant LipL32 and LipL41 antigens
Chicken anaemia virus (CAV) and Rabies antibody detection kits based on latex agglutination assays using recombinant VPI and G antigens respectively

Vaccines developed

Nanoparticles Biomarker coupled Newcastle disease virus (NDV) vaccine
Live attenuated PPRV vaccine from a local goat strain
Live attenuated and inactivated infectious bronchitis virus (IBV) vaccine using a local isolate
Inactivated cell culture adapted NDV vaccine

Process developed

Chikungunya diagnosis by reverse transcription polymerase chain reaction (PCR)
IBV diagnosis and genotyping
Rabies antibody assessment by Rapid Fluorescent Focus Inhibition Test (RFFIT)
NDV pathotyping
PPRV and CDV diagnosis by real-time PCR
Marek’s and leptospiral genome detection by loop mediated isothermal amplification assay
Avian oncogenic virus genome detection and differentiation by molecular techniques
Diagnosis of tuberculosis and paratuberculosis by PCR
Leptospira count and typing by a single tube real-time PCR assay
Eimeria speciation by PCR and preponderance by real-time PCR
Derivatization and characterization of mesenchymal stem cells from sheep foetal bone marrow and buffalo adipose tissue

Research in Social Sciences, Agriculture and IT

Developed e-Courses (both On-line and Off-line) for BVSc & AH Degree programme
Formulated ‘Milk Price Policy Model’ for Tamil Nadu to revise and update milk procurement price periodically
Developed “Technology Achievement Index” for Livestock Sector
Evaluated the impacts and impediments of Animal Husbandry development schemes of Government of Tamil Nadu
District Agricultural Plan for 31 districts in Tamil Nadu for implementing/funding schemes under Rashtriya Krishi Vikas Yojana was drafted
Economic losses due to conception failures, FMD and metabolic disorders in bovines were estimated
Biotech Kisan hub at TNAU and TANUVAS
Water conserving hydroponic green forage production for livestock farming
Fodder cultivation technologies
Probiotic bacteria identified in elephant, tiger, sloth bear, Nilgiri langur and reptiles
Cortisol levels estimated in wild animals
Molecular species identification in reptiles and mammals

Patents Granted (7)

A test strip for qualitative detection of antibodies to peste des petits ruminant virus (Patent No. 243290 dated 04.10.2010)
ABT mini encapsulator Patent No. 347388 dated 24.09.2020
An In-vitro molecular method for identifying gender in Emu Patent No. 349823 dated 22.10.2020
Nano-Biomarker coupled Newcastle disease vaccine Patent No. 349908 dated 22.10.2020
A novel method to diagnose small ruminants with potential resistance to blue tongue Patent No. 353468 dated 11.12.2020
Multiplex PCR primers for detection of fowl oncogenic viruses Patent No. 379484 dated 20.10.2021
Genotype specific cell culture vaccine for Newcastle disease Patent No. 391161 dated 04.03.2022

Technologies Commercialised (12: Rs. 25.70 lakhs)

S. No.	Technology	Product / Technology Transferred to industries/ Field Use	Revenue Generated
1.	LeptoMultiEp-IgM ELISA based assay for serodiagnosis of Leptospirosis using novel recombinant multiepitopic antigen technology	M/s. Genomix Biotech, Hyderabad	Rs. 3,00,000/-
2.	EndoMetB PB-A probiotic consortium developed for use as a bovine uterine supplement in mild and moderate bovine endometritis	M/s. Genomix Biotech, Hyderabad. through TRPVB	Rs.5,00,000/-
3.	ABT-CHOICE -The kit used for antibiotic selection for mastitis treatment	M/s. Genomix Biotech, Hyderabad	Rs.5,00,000/-
4.	Emu Sexing Kit	VR3 Emu Farms and Hatcheries, Puducherry	Rs.6,00,000/-
5.	TANUCHEK, a field based test kit for quantification of somatic cell counts in milk and used for early detection of mastitis	Supplied for field use through the Directorate of Animal Husbandry and Veterinary Services, Government of Tamil Nadu and TRPVB, TANUVAS	Rs.1,50,000/-
6.	PPRV truncated nucleoprotein antigen	Indian Immunologicals, Hyderabad	Rs.2,00,000/-
7.	Leptospira LipL41 clone	Indian Immunologicals, Hyderabad	Rs.2,00,000/-
8.	Immunology Teaching kits	M/s. Medox Biotech, Chennai	Rs.2,70,000/-
9.	PPRV Blocking ELISA kit	Indian Immunologicals, Hyderabad	Only royalty
10.	Leptospira Latex Agglutination kit	M/s. Fort Pharma, Chennai	Rs.1,00,000/-
11.	ABT Detect, a screening assay kit for detection of antibiotic residues in meat/milk	Transferred to TRPVB, TANUVAS for commercialization	Rs.1,50,000/-
12.	Aural Spray for Dog to reduce the odour	Transferred to TRPVB, TANUVAS for commercialization	Rs.1,00,000/-

Other achievements

Sequencing the whole genome of five local isolated of NDV (HQ011508, FJ986192, GU187941, HQ902590, HM357251)
Expression profiling and ligand-induced cytokine profiles of toll-like receptors (TLRs) in Toda buffaloes and local breeds of goats
Ratite sexing methods by PCR and real time PCR
Elucidating the role of interferon alpha in the species-specific resistance of PPR
Signaling lymphocyte activation molecule (SLAM) is a coreceptor for PPRV
Expressed sequence tags of buffalo genome (335), Eimeria ¬(283) and sharks (2051)
Molecular epidemiology of NDV, IBV, PPR, CAV, Marek’s disease and Infectious bursal disease viruses