MADRAS VETERINARY COLLEGE

DEPARTMENT OF ANIMAL BIOTECHNOLOGY

Genesis

The Department of Animal Biotechnology was started in the year 1989. The Indian Council of Agricultural Research (ICAR) recognized the department as a "Centre of Excellence in Veterinary Biotechnology and Immunology" in the year 1999. It is the first Animal Biotechnology Department in this country that was awarded the ISO 2001:9000 for Quality Management Systems through the British Standards Institute, UK. The Department has been awarded second term in “Niche Area Scheme of Excellence in Avian Biotechnology” to conduct research on "Improved and expanded vaccines and Immunological understanding of avian viruses" by ICAR. Since 1989 the dept has completed nearly 85 externally funded schemes from various funding agencies like, DBT, DST, DRDO, ICAR, TANII, SLURB, NADP.

The department has an excellent track record in publications and placement with its post graduate students finding positions within India and globally.The department has been granted with 8 Patents and commercialized 18 technologies to various agencies, developed 4 vaccines.
The Rabies Diagnostic Laboratory of Department of Animal Biotechnology is an ISO/IEC 17025:2017 accredited Laboratory to carry out testing for rabies antigen by florescent antibody assay and Antibody titration by virus neutralization assay. Recently, the department is supported by DST- FIST Grant in the field of “Animal Health, Food Safety and Production” since 2021.

Objectives

To provide education and training in all spheres of Biotechnology
To carryout research in various areas of biotechnology to ultimately improve animal health, production and productivity
To disseminate the research technologies developed, to veterinarians, research scientist, students, entrepreneurs and commercial establishments.

Education

The Department offers following Post Graduate (PG) courses

M.V.Sc & Ph.D in Veterinary Biotechnology
M.Sc. and Ph.D. in Biotechnology for life science and engineering students.
PG diploma in Advanced Reproductive Biotechnology in Animal Model (Distance learning mode)
PG Diploma in Molecular Biotechniques (PGDMBT)(Regular mode)

Research

List of Completed Projects (2020 – 2025)

S.No	Title of the project	Funding agency	Budget (Rs in Lakhs)
1	Selective isolation of sex specific spermatozoa in bovines using novel biomarkers identified through an integrated proteomic and Genomic approach	DBT, GOI	91.33
2	Developing Portable instrumentation for the existing technologies available at TANUVAS for detection of post- partum disease conditions in bovines	DST, New Delhi	21.80
3	Bioelectricity and biohydrogen production using slaughter house waste rumen fluid.	TANII	19.86
4	Collaborative Research Project on “Accelerating bovine Tuberculosis Control in Developing Countries – India (ABTBCD)”	Pennsylvania State University, USA	USD 325111
5	Bovine tuberculosis control: Mycobacterial disease in animals network (MyDAN) programme	DBT, New Delhi	200.00
6	Isolation, lineage characterization and pathogenicity testing of street rabies virus	Reliance Life Sciences	16.68

On-going Research Projects

S. No.	Title of the project	Funding agency	Budget (Rs in Lakhs)
1.	Level I in the Broad Subject Area on “Animal Health, Food Safety and Production”	DST-FIST New Delhi. 5 years	300.00
2.	Augmenting reproductive and production potential through adoption of assisted reproductive techniques using sexed semen in dairy cattle	NADP, 5 years	75.71
3.	Ultra-Sensitive and Highly Specific Padlock Probe Assay for Point-of-Care Ante Mortem Rabies Diagnosis	DBT, 3 years	41.91
4.	Surveillance, Molecular Characterization and Development of Vaccine Candidate for Porcine Parvovirus	NLM, 3 years	45.29
5.	Developing Immune Complex Vaccine for Protection against Infectious Bursal Disease in the presence of Maternally Derived Antibodies	DBT, 2 years	22.55
6.	Genetic Characterization and Vaccine Delivery System for Rabies in Animals	ICMR, 3 years	47.79
7.	ICMR funded Scheme on “Sweep Monitoring of Antimicrobial Resistance Threats (SMART) in Animal-Human interface areas: One Health Approach”	ICMR, 3 years	73.15
8.	Novel Functional feed additives using biogenics to enhance broiler production	DST, 3years	42.62

Services Offered

I. Human Resources Development Programmes offered for Life science and Engineering Students

HRD Programme for training in various fields of Biotechnology such as Molecular Biology, Immunotechnology including Environmental biotechnology and Nanotechnology, Animal Cell Culture and Embryo Biotechnology.

S. No.	Training Programme	Training Period		Training Fees (Incl. 18% GST)
1.	HRD level I Training	5 days		Rs. 9,440/-
2.	HRD level II Training	II a	28 days	Rs.15,340/-
		II b	21 days	Rs.11,800/-
		II c	14 days	Rs.8,850/-
3.	HRD level III Training	III a	3 months	Rs.23,600/-
		III b	> 3 - 6 months	Rs.35,400/-
		III c	> 6 - 9 months Projects	Rs.47,200/-
		III d	> 9 - 12 months projects	Rs.59,000/-

II. Human Resource Development Training Programmes in the field of Assisted Reproductive Biotechnology

The following training programmes pertaining to Reproductive Biotechnology are offered at the Centralized Embryo Biotechnology Unit, Madhavaram Milk Colony Campus every year to cater to the needs of the Teaching / Research staff working in Veterinary / Medical / Pharmacy colleges / Scientific institutions with a basic degree of M.B.B.S., / B.V.Sc., / B. Pharm., / B.Tech., / B.Sc., or any equivalent degree in life sciences

a) National Level Training

S. No.	Training Programme	Number of days	Training Fees (Incl. 18% GST)
1	National level training programme on “In vitro fertilization of farm animal oocytes and co-culture” for 21 days for Veterinarians, Medical Professionals and life science graduates seeking a career in embryology	21 days	Rs.64,900/-
2	National level training programme on “Intra cytoplasmic sperm injection of farm animal oocytes” for two weeks for Veterinarians, Medical Professionals and life science graduates seeking a career in embryology	14 days	Rs.94,400

b) Short-Term IVF Training programmes

The Department of Animal Biotechnology offers Short term IVF training programme pertaining to Reproductive Biotechnology at the Centralized Embryo Biotechnology Unit, Madhavaram Milk Colony Campus to cater to the needs of the students pursuing M.B.B.S, / B.V.Sc & AH. / B. Pharm. / B.Tech. / B.Sc. or any equivalent degree in life sciences. Three modules of the training programme are available of varying duration so that students can choose according to their interest and research needs.

S. No.	Training Programme	Number of days	Training Fees (Incl. 18% GST)	Contents of the training programmes
1.	Module I: In vitro embryo production - Handling of female gamete	5 days	Rs.6,962/-	1. Introduction to female reproductive anatomy and oogenesis 2. Aseptic techniques and laboratory equipment’s for IVF 3. Media preparation 4. Oocyte retrieval from slaughter house specimens 5. Oocyte culture and Grading
2.	Module II: In vitro embryo production - Handling of male gamete	5 days	Rs.6,962/-	1. Introduction to male reproductive anatomy and spermatogenesis 2. Aseptic techniques and laboratory equipments for IVF 3. Media preparation 4. Sperm retrieval from frozen semen straws 5. Preparation of sperms and IVF
3.	Module III: In vitro embryo production - Handling of female and male gametes (combined)	10 days	Rs.10,620/-	1. Introduction to female reproductive anatomy and Oogenesis 2. Aseptic techniques and laboratory equipments for IVF 3. Media preparation for oocyte culture 4. Oocyte retrieval from slaughter house specimens 5. Oocyte culture and Grading 6. Introduction to male reproductive anatomy and spermatogenesis 7. Media for sperm preparation 8. Sperm retrieval from frozen semen straws 9. Preparation Of sperms for culture 10. Determination of sperm count, sperm viability followed by IVF

IV. Biotechnological services offered on cost basis

Biotechnology Service offered	Cost Per Sample (Excl. GST)
Rabies antibody detection in serum from animals by FAVN (NABL Accredited Rabies Diagnostic Laboratory)	Rs. 250
Rabies antigen detection by DFAT	Rs. 100
Diagnosis by PCR	Rs. 900
Diagnosis by Reverse Transcriptase PCR	Rs. 1000
Real time quantitative PCR	Rs. 1200
Genotyping of avian viruses by PCR-RFLP	Rs. 3600
Nucleic acid and protein quantification	Rs. 100
Agarose Gel Electrophoresis	Rs. 250
Gel documentation AGE& SDS-PAGE	Rs. 250
Supply of a cell line flask	Rs. 750
Usage of cell culture facility (Cytotoxic experiments)	Rs. 1500
Photomicrography	Rs. 100
Detection of Infectious bronchitis virus by reverse transcription PCR and its genotyping by sequencing	Rs. 2200
Zeta potential	Rs. 500
Particle size analyser	Rs. 500
Standard Plate Count (Aerobic)	Rs. 500
Cell adhesion property analysis	Rs. 1500
Bile salt tolerance analysis	Rs. 200
Testing the contamination of E.coli, Staphylococcus sp., Yeast and Mold	Rs. 400
Bacterial species identification by PCR and sequencing	Rs. 2000
Cyclic Voltametry	Rs. 100
Detection of Feline herpes virus (FPV) by PCR	Rs. 750
Detection of Feline Leukaemia virus by RT-PCR	Rs. 900
Detection of Feline Coronavirus (FCoV) by RT-PCR	Rs. 900
Atomic Absorption Spectrometry (AAS) – Flame Method Lead* (Pb), Chromium* (Cr), Copper (Cu), Sodium (Na), Potassium (K), Calcium (Ca), Zinc (Zn), Selenium (Se)	For any one, two, three, four, five, six, seven & eight mineral– Rs. 300/-,Rs. 500/-,Rs. 750/-, Rs. 1000/-, Rs. 1200/-, Rs. 1350/-, Rs. 1400/- &Rs. 1500/-
Atomic Absorption Spectrometry (AAS) – Graphite Furnace Method Lead* (Pb), Chromium* (Cr), Copper (Cu), Sodium (Na), Potassium (K), Calcium (Ca), Zinc (Zn), Selenium (Se)	For any one, two, three, four, five, six, seven & eight mineral– Rs. 400/-,Rs. 600/-,Rs. 850/-, Rs. 1100/-, Rs. 1300/-, Rs. 1500/-, Rs. 1700/- &Rs. 1800/-

VI.Instrument Facilities

The department has six well established laboratories with instruments such as Gradiant Thermalcycler, Real time PCR, Atomic absorption spectrophotometer, nanodrop, ELISA reader, Gel Documentation, CO2 Incubator, Fermentor, Gas liquid chromatography, Potentiostat, and Fluorescent inverted microscope.

NABL accreditation of Rabies Diagnostic Laboratory:

The ISO/IEC 17025:2017 accreditation of Rabies Diagnostic Laboratory is renewed for the period of four years from 08.11.2024 to 07.11.2028

Patent Granted

Name of the invention	Year	Patent Number
A test strip for quantitative detection of antibodies to PPRV	04.10.2010	243290
Nano-biomarker coupled Newcastle disease vaccine (NDV)	22.10.2020	349908
A novel method to diagnose small ruminants with potential resistance to bluetongue	11.12.2020	353468
Anin-vitromolecular method for identifying gender in emu	22.10.2020	349823
Chicken Anaemia Virus (CAV)Antibody Detection Kit	22.09.2020	347388
ABT miniencapsulator	24.09.2020	347623
Multiplex PCR primers for Detection of Fowl Oncogenic Viruses	20.10.2021	379484
Genotypic specific cell culture vaccine for newcastle disease	30.09.2023	391161

commercialized Products

S. No.	Name of the Technology Developed	Product / Technology Transferred to industries/ Field Use
1.	LeptoMultiEp-IgM ELISA for serodiagnosis of Leptospirosis using novel recombinant multiepitopic antigen technology	M/s. Genomix Biotech, Hyderabad
2.	EndoMetB PB-A probiotic consortium for use as a bovine uterine supplement in mild and moderate bovine endometritis	M/s. Genomix Biotech, Hyderabad.
3.	ABT-CHOICE - for antibiotic selection for mastitis treatment	M/S Immunoshop, Hyderabad
4.	Emu Sexing Kit	VR3 Emu Farms and Hatcheries, Puducherry
5.	TANUCHEK, a field based test kit for quantification of somatic cell counts in milk and used for early detection of mastitis	Directorate of Animal Husbandry and Veterinary Services, Government of Tamil Nadu and TRPVB, TANUVAS
6.	PPRV truncated nucleoprotein antigen	Indian Immunologicals, Hyderabad
7.	Leptospira LipL41 clone	Indian Immunologicals, Hyderabad
8.	Immunology Teaching kits	M/s. Medox Biotech, Chennai
9.	PPRV Blocking ELISA kit	Indian Immunologicals, Hyderabad
10.	Leptospira Latex Agglutination kit	M/s. Medox Biotech, Chennai
11.	ABT Detect, a screening assay kit for detection of antibiotic residues in meat/milk	TRPVB, TANUVAS
12.	Aural Spray for Dog to reduce the odour	TRPVB, TANUVAS
13.	MYCO-ID TB detection kit was transferred	TRPVB, TANUVAS

Diagnostic kits & Vaccines Developed

Multiple PCR for detection and differentiating of M. tuberculosis and M. bovis and M. orygis
Nanoparticle Biomarker coupled Newcastle disease virus (NDV) vaccine
Live cell culture adapted PPR vaccine from a goat strain
Inactivated cell culture-adapted Newcastle disease vaccine
TANUVAS Infectious bronchitis vaccine for poultry

Faculty

Name of the Faculty	Designation	E-mail	Mobile#
PARTHIBAN,M	PROFESSOR AND HEAD	drparthiban66@yahoo.com	9600562996
RAJA,A	PROFESSOR	raja.a@tanuvas.ac.in	9444140452
MEIGNANALAKSHMI,S	PROFESSOR	meignanalakshmi.s@tanuvas.ac.in	9884317730
BRINDHA,K	PROFESSOR	brindhanarayanan@gmail.com	9840971881
KALAISELVI,G	ASSISTANT PROFESSOR	kalaiselvi.g@tanuvas.ac.in	8526885590
PARTHIBAN,S	ASSISTANT PROFESSOR	parthiban.s@tanuvas.ac.in	9944219889
VIJAYANAND,J	ASSISTANT PROFESSOR	vijayanandj6@gmail.com	90471 57859
RAJA,P	ASSISTANT PROFESSOR	raja.p@tanuvas.ac.in	9710316741

The Professor and Head,
Department of Animal Biotechnology,
Madras Veterinary College,
Chennai – 600 007.
Contact No. 9600562996
E-mail: hodabtmvc@tanuvas.org.in